Laboratory for Biological Ultrastructure

The Department of Biology at the University of Maryland, College Park maintains the Laboratory for Biological Ultrastructure, a high-quality, well-equipped biological imaging facility. For more than 30 years, this facility has been an important resource for university faculty and students. The multi-room supervised facility contains two transmission (Zeiss EM 10 CA and JEOL 100CX II) and two scanning (Hitachi S-4700 and Amray 1820D) electron microscopes. Light microscopic instrumentation includes two Zeiss photomicroscopes equipped with bright field, phase contrast, differential interference contrast and polarized light optics. A recently acquired Bio-Rad MRC 1024 confocal microscope has been added to provide fluorescent and laser scanning capabilities. Although the laboratory is housed in and funded by the College of Computer, Mathematical, & Natural Sciences, it is open to use by other researchers of the university community. Investigators from the following departments benefit from the facility's state-of-the-art equipment:

  • Animal and Avian Sciences
  • Biology
  • Cell Biology and Molecular Genetics
  • Chemistry and Biochemistry
  • Chemical Engineering

For more information:
Laboratory for Biological Ultrastructure
Timothy Maugel, Director
0240 Biology-Psychology Building
Phone: 301.405.6898
Fax: 301.314.9358

Zeiss EM 10 CA transmission electron microscope
Equipped with a tilt stage and Dage 68 high-resolution analog video camera.

JEOL 100CX II transmission electron microscope
Equipped with a scanning attachment.

Amray 1820D scanning electron microscope, LaB6 source
Equipped with a EDAX Genesis energy dispersive x-ray microanalysis 
system, Robinson backcattered electron detecto, Robinson Chamber
View camera system, Pic I digital image aquisition system and a 
Seikosha video printer.

Hitachi S-4700 scanning electron microscope, field emission source
Equipped with Hitachi cryo-stage, Centaurus backscattered electron
detector and GW chamberscope camera.

Bio-Rad MRC 1024 laser confocal imaging system on a Nikon
Diaphot 300 inverted microscope

Equipped with DIC and fluorescent optics.

Two Zeiss Photomicroscope II
Equipped with bright field, Normarski differential interference, phase contrast
and polarizing optics.

Two Zeiss RA compound microscopes and five Wild and Bausch & Lomb
dissecting microscopes
Preparative paraphernalia includes both Balzers 400T and Denton freeze fracture devices. Four ultramicrotomes(one of them a Reichert Ultracut E equipped for cryosectioning), vacuum evaporators, a chromium coater. microwave fixation apparatus, propane jet and plunge cryofixation apparatuses, freeze drying and critical point drying instruments. In addition, a full complement of general instrumentation including balances, centrifuges, pH meter, 
osmometer, shakers, dissecting and compound microscopes, ect., is maintained in operating condition within the Laboratory.
Imaging Recording and Analysis Instrumentation 

Light microscopic images can be recorded in film(35mm or sheet film), video or digital formats. High resolution flatbed and slide scanners are available for converting analog images to digital format. Video, laser, inkjet, thermal and dye-sub printers are available to provide photographic prints of images from LM, SEM or TEM. Complete wet photographic facilities, including film and plate processing, print production on a point source enlarger, and trimming and mounting equipment are contained within the facility for the preparation of publication ready material.